BIO306 (Genetics)
Drosophila Research
Paper --- Specific Guidelines
(with links to general guidelines)
NOTE:
Also consult the grading rubric when writing your paper. If the rubric
contradicts any of the guidelines presented on this page, the rubric takes precedence over this page.
General Format
The paper should be divided into sections
that are placed in the following order:
-
Title
-
Abstract
-
Results
-
Discussion
-
Literature Cited
Sections should be labeled (e.g., ABSTRACT
or Abstract or Abstract to set them apart from the text)
The text should be double-spaced. Information
in tables and the Literature Cited can be single-spaced.
NOTE that you are not writing all of the sections of a
primary literature paper. For example, you will NOT write an Introduction or a
Materials and Methods sections. However, to help you better understand how your
paper should sound, please follow this link to a sample Materials and Methods
section. Read this document and try to mimic its quality and tone while
writing the required sections for your paper.
Watch plagiarism:
copying someone else's words, even if you reference their work, is illegal.
You must put everything into your own words, and then if those ideas that
you are describing are someone else's, you also must cite the reference
from which those ideas came. Do not quote someone's words as you might
do in a history or literature paper; scientists don't do that in standard
research papers.
Consult the Communication in Biological
Sciences website for two methods of
citing references, choose one of those two, and consistently and correctly
use them throughout your paper.
Writing should be in the past tense and
third person:
-
Yes: "The flies were counted…"
-
No: "I counted the flies…"
-
No: "Count the flies…"
Proper grammar and punctuation should be used
throughout; spelling is also important. Microsoft Word has a pretty good
grammar check, and although you cannot necessarily trust the spell check,
it’s better than not checking at all.
However, YOU should read through both the
initial and final drafts of your paper before handing them in. Critiquing
your own work is very difficult, but it is a skill that will serve
you well no matter what you end up doing.
Keep in mind that the writing and flow of your paper is
important. Simply stringing together all of the items listed below for each
section is NOT ENOUGH.
Text
TITLE
ABSTRACT
-
Includes a short and concise explanation of
what question you addressed in the experiment. For example: one sentence that
introduces the question that was addressed, one sentence that states the methods
(virgin female mutant flies were crossed with wild-type males...), one sentence
that reports the results, and one sentence that makes a conclusion based on the
original question that was addressed.This is generally the last section
that you write because it is a summary. However, some writers like to write the
abstract first because it is like a mini version of the paper and helps to organize
their thoughts (some editing of the abstract is usually required after the rest of the paper
is finished, but it's worth it if this method works for you).
RESULTS
-
In a sentence or two, recap the fly cross experiment by stating the
hypothesis that was being tested and the general overview of the crosses that
were done
-
Begin with a description of the phenotypes
of the F1 flies
-
Write out the F2 results in the text (remember
that numbers 10 and over are written as numbers whereas numbers under
10 are written out as words unless the numbers are part of a ratio)
-
Include the F2 results in a table;
remember to reference the table in the text
- Calculate the observed ratio based on your
data (this will probably not be exactly a 3 wild-type:1 mutant, for example)
and include this information in the text
-
Explain that a Chi-square test was done and
why, and what the Chi-square value was
-
If you include other figures
and/or tables, be sure
to reference them properly in the text
-
Do not include any interpretation of the results
in this section!!
DISCUSSION
-
In a sentence or two, recap the experiment by stating the
hypothesis that was being tested and the general overview of the crosses that
were done
-
Interpret the Chi-square value
-
State the critical value and the p value(s)
associated with your Chi-square value
-
Conclude whether the hypothesis is rejected
or supported by the data and explain why you concluded that based on your
Chi-square analysis (include the p values in your explanation)
-
If your hypothesis is not rejected, list one
or two other experiments that could be done to continue your work
-
If your hypothesis is rejected, come up with
one or more reasons why (e.g., the genes you are working with are linked,
all your F2 winged flies flew away, etc.), state what you would do differently
next time, list one or two other experiments that could be done to continue
your work
-
You set up your initial Parental cross by crossing
females of one type and males of another. Discuss the results that you would
have expected had you done the reciprocal cross. (Would they have been the same
or different and why?)
-
Discuss the wild-type function(s)
of your fly gene(s) (with references), at the functional, molecular level. What type of protein does each
gene encode? Enzyme? Transport protein? Receptor? Signaling protein? Structural protein? Transcription factor? etc.
How does loss of this function lead to the phenotype of your mutant strain?
-
Finish the paper with some sort of summary of your
findings
LITERATURE
CITED
-
Be sure that you format these correctly and
include all of the information necessary
-
You need at least four references for this
paper, one of which must be primary literature
-
If you use the lab manual, your text, or the
Carolina Drosophila manual, please realize that these three sources
are BOOKS. Click on the Literature Cited link just above, go to the Examples,
and look at Examples of book citations to see how they're done.
-
Remember that if a source has more than one
author, that when cited, the order of those authors must not change.
IMPORTANT: Before you turn in your
paper, you need to revise it many times. Please use the following website
for tips on reviewing a manuscript.